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KMID : 0900920010250010085
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Korean journal of Animal Reproduction
2001 Volume.25 No. 1 p.85 ~ p.90
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Transmission and Death Rates in Transgenic Mice Containing Growth Hormone Receptor Gene
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Kim H.-J.
Jin Dong-Il
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Abstract
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To study the signaling effect of growth hormone (GH) in vivo on animal physiology, transgenic mice containing GH Receptor (GHR) gene fused to metallothionein promoter were produced by DNA microinjection into one-cell stage embryos. Three founder mice were produced with transgenic mice with approximately 4~6 copies of GHR genes and transgene was transmitted into the progeny. The founder mice were mated with normal mice to produce F_1 mice, and intergation and transmission of transgene were checked by polymerase chain reaction and Southern blot methods. Transmission rate of GHR transgenic mice were 20~50% in F_1 generation and 50% in F_2 generation which means that some founder mice were mosaic and transgene in F_1 mice was transmitted to F_2 progeny with Mendelian ratio. Death rate of GHR transgenic mice after birth was about 10~30% in F_1 and F_2 progenies indicating that GHR gene may affect death of transgnenic progeny.
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KEYWORD
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